Plasmid

Part:BBa_K4344043:Design

Designed by: Marcel Pott   Group: iGEM22_Heidelberg   (2022-09-29)


pRK5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 919
    Illegal XbaI site found at 936
    Illegal SpeI site found at 28
    Illegal PstI site found at 948
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 919
    Illegal SpeI site found at 28
    Illegal PstI site found at 948
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 919
    Illegal BamHI site found at 930
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 919
    Illegal XbaI site found at 936
    Illegal SpeI site found at 28
    Illegal PstI site found at 948
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 919
    Illegal XbaI site found at 936
    Illegal SpeI site found at 28
    Illegal PstI site found at 948
    Illegal NgoMIV site found at 1908
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

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Source

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References